| Facility | Hogwarts |
| Analyst | John Doe |
| Analysis started | 2025-03-19 23:05:04 |
| Analysis completed | 2025-03-19 23:05:06 |
| Wall time | 0:0:2 hours |
| locus | COI |
| preliminary_id | Miridae |
| taxa_of_interest |
Lygus pratensis |
| country | Netherlands |
| host | Cut flower Paenonia |
| sample_id | VE24-1079_COI |
| Query DNA sequence |
Inconclusive
The analyst should attempt subjective species identification at the genus level.
Reasoning - Flag 1B:
2-3 candidate species matched with high stringency (identity ≥ 98.5%).
| Preliminary morphology ID confirmed | NA |
|
Inconclusive taxonomic identity (Flag 1B) |
|
| Taxa of interest ruled out | False |
|
Flag 2A: Taxon of interest NOT detected Flag 5.1A: The given locus for this taxon is well represented in reference database (>5 entries) Flag 5.2B: 10-90% of related taxa have reference sequence(s) at the given locus |
|
Flag 1B:
The analyst should attempt subjective species identification at the genus level
2-3 candidate species matched with high stringency (identity ≥ 98.5%)
Candidate hits must meet ONE of these criteria:
| Minimum alignment length |
400bp
|
| Minimum query coverage |
85.0%
|
Candidate hits are then classified as follows:
| Classification | Alignment identity | Number of hits | Number of species |
|---|---|---|---|
| STRONG MATCH | ≥ 98.5% | 11 | 2 |
| MODERATE MATCH | ≥ 93.5% | NA | NA |
| NO MATCH | < 93.5% |
Hits per candidate species (top 10 candidates only)
| Species | Hits | Identity | E-value |
|---|---|---|---|
| Orius laevigatus | 10 | 99.1% | 0.0 |
| Orius horvathi | 1 | 98.8% | 0.0 |
This sections shows the taxa of interest specified by the submitter. Each of these taxa has been cross-referenced against the candidate species to determine if they might match the taxonomic identity of the sample. A blank row indicates a TOI that did not match any candidate species, meaning that it is unlikely that the sample matches that TOI.
| Taxon of interest | Match rank | Match taxon | Match species | Match accession | Match identity |
|---|---|---|---|---|---|
| Lygus pratensis | - | - | - | - | - |
See the Database coverage section to see database coverage for taxa of interest.
This analysis evaluates how many independent sources have contributed to reference sequences for each candidate species. This provides a measure of confidence in the taxonomic annotation of references sequences. A sequence that has been annotated by multiple independent sources is more likely to have a correct taxonomic annotation.
Independent sources
Flag 4B:
Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources
(found 5 sources)
5 Independent Sources
The matching reference sequences for this species have been annotated by 5 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG007857 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007857 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
| MG007859 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007859 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
| MG007856 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007856 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
| MG007858 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Presence sur l'ile de la Reunion de deux especes de punaises predatrices potentiellement utilisables pour la lutte biologique : Orius naivashae (Poppius 1920) et Cyrtopeltis callosus Odhiambo 1961 (Hemiptera, Heteroptera : Anthocoridae et Miridae) | Bull Soc Entomol France 123 (1), 29-42 (2018) |
| MG007858 | False |
Streito,J.-C. Fontaine,O. Atiama,M. Genson,G. Pierre,E. Frago,E. Nibouche,S. |
Direct Submission | Submitted (27-SEP-2017) CBGP, INRA, 475 avenue campus agropolis. Campus international de baillarguet, Monteffrier sur lez 34980, France |
Source 2
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| FM210187 | False |
Pasquer,F. Pfunder,M. Frey,B. Frey,J.E. |
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures | Biocontrol Sci Technol (2010) In press |
| FM210187 | False | Pasquer,F. | Direct Submission | Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND |
| FM210184 | False |
Pasquer,F. Pfunder,M. Frey,B. Frey,J.E. |
Microarray-based genetic identification of beneficial organisms as a new tool for quality control of laboratory cultures | Biocontrol Sci Technol (2010) In press |
| FM210184 | False | Pasquer,F. | Direct Submission | Submitted (08-SEP-2008) Pasquer F., Molecular Diagnostics and Epidemiology, Agroscope changins Waedenswil, Schloss, Postfach 185, 8820/ Waedenswil, SWITZERLAND |
Source 3
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| KJ467500 | False |
Pinol,J. Mir,G. Gomez-Polo,P. Agusti,N. |
Universal and blocking primer mismatches limit the use of high-throughput DNA sequencing for the quantitative metabarcoding of arthropods | Mol Ecol Resour 15 (4), 819-830 (2015) |
| KJ467500 | False |
Pinol,J. Mir,G. Gomez-Polo,P. Agusti,N. |
Direct Submission | Submitted (18-FEB-2014) Entomology, IRTA (Institute for Food and Agricultural Research and Technology), Carretera de Cabrils, Km. 2, Cabrils, Barcelona 08348, Spain |
Source 4
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| GQ292147 | False |
Jung,S. Duwal,R.K. Lee,S. |
COI barcoding of true bugs (Insecta, Heteroptera) | Mol Ecol Resour 11 (2), 266-270 (2011) |
| GQ292147 | False | Jung,S.H. | Direct Submission | Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea |
| GQ292148 | False |
Jung,S. Duwal,R.K. Lee,S. |
COI barcoding of true bugs (Insecta, Heteroptera) | Mol Ecol Resour 11 (2), 266-270 (2011) |
| GQ292148 | False | Jung,S.H. | Direct Submission | Submitted (17-JUN-2009) CALS, Insect Biosystematics, Seoul Nat. Univ. 200-6102, Seoul, Korea |
Source 5
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG452375 | False |
Erfanfar,D. Srafrazi,A. Nouri- Ganbalani,G. Ostovan,H. Shojaei,M. |
COI-based identification of Orius species (Hemiptera: Anthocoridae) from Iran | Unpublished |
| MG452375 | False |
Erfanfar,D. Sarafrazi,A. |
Direct Submission | Submitted (05-NOV-2017) Department of Entomology, Science and Research Branch, Eslamic Azad University, Daneshgah Bulvar, Hesarak, Tehran 14155-4933, Iran |
Independent sources
Flag 4B:
Reference sequence sources lack diversity and may therefore be unreliable
Reasoning: Matching sequence records for this species have only 1-5 independent sources
(found 1 sources)
1 Independent Source
The matching reference sequences for this species have been annotated by 1 independent source(s). A source is considered independent if the author list or publication title is distinct.
Source 1
| Hit accession | Automated | Authors | Title | Journal |
|---|---|---|---|---|
| MG452387 | False |
Erfanfar,D. Srafrazi,A. Nouri- Ganbalani,G. Ostovan,H. Shojaei,M. |
COI-based identification of Orius species (Hemiptera: Anthocoridae) from Iran | Unpublished |
| MG452387 | False |
Erfanfar,D. Sarafrazi,A. |
Direct Submission | Submitted (05-NOV-2017) Department of Entomology, Science and Research Branch, Eslamic Azad University, Daneshgah Bulvar, Hesarak, Tehran 14155-4933, Iran |
The target taxa include candidate species, the preliminary morphology ID, and any taxa of interest provided by the submitter. Each of these taxa are independently evaluated against the reference database to determine whether sufficient reference data exists to support identification of the target taxon. Insufficient coverage of a taxon can result in that taxon not be correctly identified as the taxonomic identity of the sample. For example, if the sample is Homo sapiens, but Homo sapiens sequences are not included in the reference database, the analysis will be unable to identity Homo sapiens as the correct taxonomic identity, and will most likely assign the closest relative with reference data as the taxonomic identity.
Database coverage
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
Flag 5.1A:
The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)
There are 19 sequences in the reference database for Orius laevigatus at the given locus COI.
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Flag 5.3A:
Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: All species in genus from country of origin have reference sequence(s) for this locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Database coverage
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
Flag 5.1A:
The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)
There are 6 sequences in the reference database for Orius horvathi at the given locus COI.
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Flag 5.3A:
Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: All species in genus from country of origin have reference sequence(s) for this locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Database coverage
Flag 5.1NA:
This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species
Flag 5.1NA:
This taxon could not be fully assessed
Reasoning: Assessment of related species is only possible for taxa at rank genus/species
There are NA sequences in the reference database for Miridae at the given locus COI.
Database coverage
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
Flag 5.1A:
The reference data supports this taxon well
Reasoning: The given locus for this taxon is well represented in reference database (>5 entries)
There are 54 sequences in the reference database for Lygus pratensis at the given locus COI.
Flag 5.2B:
The database has some support for species in this genus
Reasoning: 10-90% of related taxa have reference sequence(s) at the given locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
Flag 5.3A:
Probability that a different related species from the country of origin is the true taxonomic identity: LOW
Reasoning: All species in genus from country of origin have reference sequence(s) for this locus
/ (%) sequence records were found in the reference database for:
Number of GenBank records at locus COI
This section provides a phylogeny of the candidate reference sequences. The analyst can use this to make a subjective observation on how well the reference sequences are able to distinguish between species. If the phylogeny shows distinct clades for each species, we can be confident that the molecular data are capable of distinguishing between those species. However, if the phylogeny shows overlap between species, this reduces the capacity of the molecular data to confidently distinguish between those species. In some cases, we may see the query sequence falling outside of the adjacent species' clades, which indicates that our query species is not represented in the reference database, which could indicate a rare or novel species.
The following resources can be used to ensure that the given taxonomy is legitimate and current.
| Taxa | Database |
|---|---|
| General | GBIF |
| General | ITIS |
| Mealybugs & scale | ScaleNet database |
| Thrips | Thripswiki |
| Spider Mites | Spider Mites Database |
| Psocodea (Barklice, Booklice, and Parasitic Lice) | Psocodea Species File Online |
| Orthoptera | Orthoptera Species File Online |
| Drosophilidae | TaxoDros |
| Diptera |
Catalog of the Diptera of the Australasian and Oceanian Regions
Systema Dipterorum |
| Aphids | Aphid Species File |
| Ants |
AntWeb
AntCat |
| Lepidoptera (butterflies and moths) | The Global Lepidoptera Names Index |
| Gracillariidae (primitive moths) | Global Taxonomic Database of Gracillariidae |
| Pyralidae (pyralid moths) | Global Information System on Pyraloidea |
| Tortricidae (tortrix moths) | Tortricidae Resources on the Net |